rec31806 sars cov 2 spike glycoprotein s1  (Native Antigen Inc)

Journal: bioRxiv

Article Title: An ultra-long heavy chain bovine antibody neutralizes SARS-CoV-2 and reacts broadly with sarbecoviruses

doi: 10.1101/2025.01.03.631215

Figure Lengend Snippet: Binding curves of the eight ultra-long mAbs of the panel against different SARS-CoV-2 spike variants and forms of spike (Lineage A: trimer, full-length, S1, RBD, Alpha full-length, Beta full-length, Omicron trimer). The highest concentration of each mAb was 2.5µg/mL and the lowest was 3ng/mL

Article Snippet: Two Holstein bullocks were immunised each with 250 µg of the S1 subunit of SARS-CoV-2 spike (REC31806, Native Antigen Company) mixed with Titremax Gold Adjuvant (Sigma Aldrich).

Techniques: Binding Assay, Concentration Assay

Journal: bioRxiv

Article Title: An ultra-long heavy chain bovine antibody neutralizes SARS-CoV-2 and reacts broadly with sarbecoviruses

doi: 10.1101/2025.01.03.631215

Figure Lengend Snippet: ( A ) Schematic representation of the immunisation schedule. The process lasted for 242 days and it consisted of one prime immunisation with Lineage A S1 spike, two boosts with the same antigen, one boost with Lineage A full length spike and one boost with Lineage A trimeric spike. Blood was collected 4 and ∼8 days post immunisation. ( B ) Neutralisation assay using cow serum from the final timepoint (D242) against pseudotyped SARS-CoV-2 variants and the original SARS-CoV. The serum was able to neutralise all pseudotypes tested and ID 50 values were calculated. ( C ) Table showing the EC 50 values (µg/mL) of spike binding mAbs against different forms of Lineage A spike (trimeric, full length, S1, RBD) and other variant spikes (Alpha, Beta, Omicron (BA.5)). The mAbs are divided in ultra-longs and non-ultra-longs based on their CDRH3 length in amino-acids. ( D ) Competition ELISA of the five RBD-binding mAbs (P2, P7, 99, 103, 101) with human ACE2 against Lineage A RBD. Antibodies 99, 103 and 101 showed inhibition of ACE2 with IC 50 values of 0.03, 0.32 and 0.45 µg/mL, respectively. Antibodies P2 and P7 showed partial inhibition. Mean and SD shown on graphs

Article Snippet: Two Holstein bullocks were immunised each with 250 µg of the S1 subunit of SARS-CoV-2 spike (REC31806, Native Antigen Company) mixed with Titremax Gold Adjuvant (Sigma Aldrich).

Techniques: Binding Assay, Variant Assay, Enzyme-linked Immunosorbent Assay, Inhibition

Journal: Viruses

Article Title: Reduced Binding between Omicron B.1.1.529 and the Human ACE2 Receptor in a Surrogate Virus Neutralization Test for SARS-CoV-2

doi: 10.3390/v15061280

Figure Lengend Snippet: Information about the antigens used in the surrogate virus neutralization test for SARS-CoV-2.

Article Snippet: Omicron S1 (B.1.1.529) , Native Antigen , REC32006 , HEK293 , >95% , 16–671 , 29 , 15 f.

Techniques: Neutralization, Expressing

Journal: Viruses

Article Title: Reduced Binding between Omicron B.1.1.529 and the Human ACE2 Receptor in a Surrogate Virus Neutralization Test for SARS-CoV-2

doi: 10.3390/v15061280

Figure Lengend Snippet: Information about the antigens used in the surrogate virus neutralization test for SARS-CoV-2.

Article Snippet: Omicron S1 (B.1.1.529) , Native Antigen , REC32006 , HEK293 , >95% , 16–671 , 29 , 15 f.

Techniques: Neutralization, Expressing